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Designed with versatility, reliability, and simplicity in mind, Micotox® cartridges are used for the individual or simultaneous purification of mycotoxins for further analysis by HPLC or TLC. Purification of food and feed extracts utilizing Micotox® cartridges takes less than a minute and provides high recovery rates with excellent cleaning. Purification using Micotox® cartridges is ideal for mycotoxin analysis by high-performance liquid chromatography (HPLC) or thin-layer chromatography (TLC). Micotox® cartridges are produced by Micotox Ltda. and are fully assembled in Colombia with high quality materials produced in Europe and the United States.
Micotox® cartridge reference |
USE |
Easy-push one-step format |
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M2002 |
Purification of aflatoxins in corn for HPLC analysis. |
M2004 |
Purification of aflatoxins and zearalenone (normal) |
M2006 |
Purification of aflatoxins and zearalenone (extra) |
M2003 |
Purification of thrichothecenes (small size samples) |
M2005 |
Purification of trichothecenes (normal) |
M2007 |
Purification of trichothecenes (extra) |
M2008 |
Purification of aflatoxins and patuline (extra) |
Traditional SPE format |
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M2100 |
Purification of fumonisins |
M2200 |
Purification of ochratoxin A |
M2300 |
Purification of cyclopiazonic acid |
Pre-Elisa cartridges
Micotox
Before using your ELISA kit and in just 15 seconds this mini-cartridge selectively removes interferences from extracts of complex matrices without affecting the recovery of the mycotoxin of interest.
Available for three mycotoxins: aflatoxin, ochratoxin and DON.
MICOTOX Series 2000 Clean-Up Cartridges Immunoaffinity Columns Standards Equipment Rapid Tests
Aflacup
Fast, membrane-based ELISA test kit for the determination of aflatoxins in different substrates.
Afla-strip
Afla-strip is a rapid screening test for the qualitative determination of aflatoxins in the laboratory, feed mill or storage facility. Afla-strip detects the presence of aflatoxins in several substrates in just 10 minutes.
These inmunoreactive strips for aflatoxin determination are a sensitive, reliable, fast and easy test. They do not require refrigeration or any kind of sophisticated equipment.
ELISA Kits
In a microwell format, every plate kit is a competitive ELISA for the analysis of aflatoxin, deoxynivalenol, fumonisins, T-2 toxin and zearalenone. Through the use of a microwell reader, these tests provide accurate and fast results. Ideal for large amounts of samples.
The animal feed industry has recently been severely affected by melamine contamination in high-protein ingredients and finished feed. Melamine has been used to adulterate some ingredients in order to increase the nitrogen apparent content. Melamine can cause irreversible kidney damage in pets. In response to this problem, Micotox Ltd. has been granted exclusive distribution of the only melamine ELISA test kit available in the market. The kit is a direct competitive ELISA test and has been developed for the determination of melamine in pet food (dry and moist) and wheat gluten.
The immunoaffinity columns contain monoclonal antibodies capable of attaching and purifying mycotoxins in complex special substrates such as roasted or instant coffee, spices, cotton and beer.
The high-specificity of these antibodies allows the analyst to obtain clean extracts for the further determination of the mycotoxins by chromatographic methods (TLC, HPLC) or fluorometry.
MICOTOX Series 2000 Clean-Up Cartridges Immunoaffinity Columns Standards Equipment Rapid Tests
Micotox® standards are mycotoxin working solutions containing a guaranteed concentration of each mycotoxin. Mycotoxin standards are calibrated by UV spectroscopy according to official methodologies (AOAC International official methods).
Our mycotoxin standards can be used in conjunction with our analytical methods or with any other HPLC, GC or TLC method.
Reference |
Use |
Mycotoxin concentration |
Volume |
TLC-AF |
MICOTOX® standard for four aflatoxins by TLC |
0.4 µg/mL aflatoxin B1, 0.4 µg/mL aflatoxin G1, 0.12 µg/mL aflatoxin B2 and 0.12 µg/mL aflatoxin G2 in toluene/acetonitrile 95+5. |
4 mL |
TLC-ZEA |
MICOTOX® standard for zearalenone by TLC |
10 µg/mL zearalenone in toluene/acetonitrile 95+5. |
4 mL |
TLC-3T |
MICOTOX® standard for three toxins (aflatoxin B1, zearalenone and DON) by TLC |
0.4 µg/mL aflatoxin B1, 20 µg/mL zearalenone and 20 µg/mL deoxynivalenol in toluene/acetonitrile 95+5. |
4 mL |
TLC-4T |
MICOTOX® standard for four type-A trichothecenes by TLC |
5 µg/mL T-2 toxin, 5 µg/mL HT-2 toxin, 10 µg/mL diacetoxyscirpenol (DAS) and 10 µg/mL neosolaniol in toluene/acetonitrile 95+5. |
4 mL |
TLC-OTA |
MICOTOX® standard for ochratoxin A by TLC |
1.0 µg/mL ochratoxin A in toluene/acetic acid 99+1. |
4 mL |
HPLC-AF |
MICOTOX® standard for four aflatoxins by HPLC |
250 ng/mL aflatoxin B1, 250 ng/mL aflatoxin G1, 75 ng/mL aflatoxin B2 and 75 ng/mL aflatoxin G2 in acetonitrile. |
4 mL |
HPLC-ZEA |
MICOTOX® standard for zearalenone by HPLC |
10 µg/mL zearalenone in methanol. |
4 mL |
HPLC-DON |
MICOTOX® standard for deoxynivalenol by HPLC |
50 µg/mL deoxynivalenol in methanol. |
4 mL |
HPLC-OTA |
MICOTOX® standard for ochratoxin A by HPLC |
10 µg/mL ochratoxin A in methanol. |
4 mL |
TLC Autospotter:
For the automatic application on TLC plates of up to 18 samples simultaneously
Digital densitometry system
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The digital densitometry system offered by Micotox Ltd. is composed of a viewing cabinet, a digital camera and two different software programs. The cabinet is equipped with two sets of lamps: two visible, two short-wave UV (254 nm) and two long-wave (365 nm) lamps. These lamps are positioned at both sides of the cabinet and allow for a homogeneous illumination of the thin-layer plate. On top of the cabinet comes a high-resolution digital camera controlled by computer software. Once the digital image has been obtained, the chromatographic spots are analyzed and transformed to chromatographic peaks by a special software package (peaks are similar to those obtained by a HPLC).
Components of the system:
• Viewing cabinet with flexible curtain for easy access
• Light: visible, short-wave UV (254 nm) and long-wave UV (365 nm)
• High-resolution digital camera
• Software for imaging the plates
• Software for analysis of the chromatographic spots
PHRED
Photochemical Reactor for Enhanced Detection
Analysis of aflatoxins using HPLC post-column photochemical derivatization has now been approved as AOAC official method 2005.08.
The eluent from the HPLC column is passed through a photochemical reactor “PHRED” where the aflatoxins B1 and G1 are transformed to fluorescing derivatives and then are quantitatively detected by a fluorescence detector.
The apparatus has no moving parts, is compact, reliable, no reagents are required, is simple to install and to use and is affordable. Most analysts prefer the photochemical derivatization method to that of derivatization by electrochemical bromine or post-column iodine. Besides its use for the analysis of aflatoxins, PHRED has many more applications (http://aura-inc.com/1phredapp.html).
A knitted reactor coil (KRC) is required in addition to PHRED. The specific KRC recommended for aflatoxin analysis is the KRC 25-25.